Coding
Part:BBa_K4188014
Designed by: Tobias Bröker Group: iGEM22_WWU_Muenster (2022-09-30)
AgtGPPS2 fused to PTS1 tag for the peroxisomal expression
This part is part of the genes needed to replicate the mevalonate pathway in the peroxisome. For this purpose, all required genes have been assembled and can be found in the table below. The gene is MoClo (RFC1000) compatible. The AgtGPPS2 synthesizes from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) the geranyl diphosphate (GPP).
Genes for the mevalonate pathway in the peroxisome
part number | Gene | length |
---|---|---|
BBa_K4188007 | ScERG8_PTS1 | 1365 bp |
BBa_K4188008 | ScERG19_PTS1 | 1200 bp |
BBa_K4188009 | ScERG13_PTS1 | 1485 bp |
BBa_K4188010 | ScERG10_PTS1 | 1206 bp |
BBa_K4188011 | ScIDI1_PTS1 | 876 bp |
BBa_K4188012 | ScERG12_PTS1 | 1341 bp |
BBa_K4188013 | SltNPPS1_PTS1 | 792 bp |
BBa_K4188014 | AgtGPPS2_PTS1 | 900 bp |
BBa_K4188015 | SctHMGR_PTS1 | 1518 bp |
BBa_K4188016 | GgmFPS144_PTS1 | 1113 bp |
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 332
Illegal PstI site found at 710 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 332
Illegal PstI site found at 710 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 305
Illegal BamHI site found at 495 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 332
Illegal PstI site found at 710 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 332
Illegal PstI site found at 710 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |